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Resistance to inactivation by EGTA of the enzyme-substrate and enzyme-phosphate complexes of alkaline phosphatase.

Bovine intestinal mucosal alkaline phosphatase is inactivated by the chelating agent EGTA. Several concentrations of the enzyme were incubated with EGTA and a range of concentrations of the substrate p-nitrophenyl phosphate to determine the substrate concentration as a function of time. As predicted...

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Autors principals: Pike, S J, Duggleby, R G
Format: Artigo
Idioma:Inglês
Publicat: 1987
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1148064/
https://ncbi.nlm.nih.gov/pubmed/3128267
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