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Activation of phospholipase C associated with isolated rabbit platelet membranes by guanosine 5'-[gamma-thio]triphosphate and by thrombin in the presence of GTP.

Rabbit platelets were labelled with [3H]inositol and a membrane fraction was isolated in the presence of ATP, MgCl2 and EGTA. Incubation of samples for 10 min with 0.1 microM-Ca2+free released [3H]inositol phosphates equivalent to about 2.0% of the membrane [3H]phosphoinositides. Addition of 10 micr...

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Main Authors: Hrbolich, J K, Culty, M, Haslam, R J
格式: Artigo
語言:Inglês
出版: 1987
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC1147877/
https://ncbi.nlm.nih.gov/pubmed/2820381
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