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Probing the sequence-specific interaction of the cyclic AMP receptor protein with DNA by site-directed mutagenesis.

Mutants in the DNA-binding helix of the cyclic AMP receptor protein (CRP), as well as mutants in a synthetic DNA-binding site derived from the sequence in the lac regulatory region, have been constructed by oligonucleotide-directed mutagenesis, and used to study the effect of selected amino acid sub...

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Detalhes bibliográficos
Main Authors: Gent, M E, Gronenborn, A M, Davies, R W, Clore, G M
Formato: Artigo
Idioma:Inglês
Publicado em: 1987
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1147760/
https://ncbi.nlm.nih.gov/pubmed/3109398
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