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An improved spectrophotometric assay for leucine aminopeptidase.

A sensitive assay to determine the activity of leucine aminopeptidase (EC 3.4.11.1), using L-leucine thiobenzyl ester as substrate, was developed. Hydrolysis of the ester by leucine aminopeptidase can be monitored in the presence of 5,5-dithiobis-(2-nitrobenzoic acid) by continuous spectrophotometri...

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Autors principals: Beattie, R E, Guthrie, D J, Elmore, D T, Williams, C H, Walker, B
Format: Artigo
Idioma:Inglês
Publicat: 1987
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1147694/
https://ncbi.nlm.nih.gov/pubmed/3593241
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