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Kinetics of Human Immunodeficiency Virus Type 1 (HIV) DNA Integration in Acutely Infected Cells as Determined Using a Novel Assay for Detection of Integrated HIV DNA

We have developed a novel linker-primer PCR assay for the detection and quantification of integrated human immunodeficiency virus type 1 (HIV) DNA. This assay reproducibly allowed the detection of 10 copies of integrated HIV DNA, in a background of 2 × 10(5) cell equivalents of human chromosomal DNA...

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Autors principals: Vandegraaff, Nick, Kumar, Raman, Burrell, Christopher J., Li, Peng
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2001
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC114708/
https://ncbi.nlm.nih.gov/pubmed/11602768
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.75.22.11253-11260.2001
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