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The cloning and expression of the aroL gene from Escherichia coli K12. Purification and complete amino acid sequence of shikimate kinase II, the aroL-gene product.

The aroL gene encoding the enzyme shikimate kinase II was cloned from Escherichia coli K12. Construction of over-expressing strains permitted for the first time the purification to homogeneity of a monofunctional shikimate kinase. The complete amino acid sequence of shikimate kinase II was determine...

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Detalhes bibliográficos
Principais autores: Millar, G, Lewendon, A, Hunter, M G, Coggins, J R
Formato: Artigo
Idioma:Inglês
Publicado em: 1986
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1147003/
https://ncbi.nlm.nih.gov/pubmed/3026317
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