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Overproduction of the cyclic AMP receptor protein of Escherichia coli and expression of the engineered C-terminal DNA-binding domain.
Overproduction of the cyclic AMP receptor protein (CRP) from Escherichia coli, up to 25% of the soluble cell protein, has been achieved in an inducible host-vector system under transcriptional control of the lambda promoter PL. This system is ideally suited for large scale production and purificatio...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1986
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1146894/ https://ncbi.nlm.nih.gov/pubmed/3539103 |
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