Overproduction of the cyclic AMP receptor protein of Escherichia coli and expression of the engineered C-terminal DNA-binding domain.

Overproduction of the cyclic AMP receptor protein (CRP) from Escherichia coli, up to 25% of the soluble cell protein, has been achieved in an inducible host-vector system under transcriptional control of the lambda promoter PL. This system is ideally suited for large scale production and purificatio...

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Bibliographic Details
Main Authors: Gronenborn, A M, Clore, G M
Format: Artigo
Language:Inglês
Published: 1986
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1146894/
https://ncbi.nlm.nih.gov/pubmed/3539103
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