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Biochemical and Genetic Studies of the Initiation of Human Rhinovirus 2 RNA Replication: Purification and Enzymatic Analysis of the RNA-Dependent RNA Polymerase 3D(pol)

The replication of human rhinovirus 2 (HRV2), a positive-stranded RNA virus belonging to the Picornaviridae, requires a virus-encoded RNA polymerase. We have expressed in Escherichia coli and purified both a glutathione S-transferase fusion polypeptide and an untagged form of the HRV2 RNA polymerase...

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Bibliografiske detaljer
Udgivet i:J Virol
Principais autores: Gerber, Kinga, Wimmer, Eckard, Paul, Aniko V.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2001
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC114677/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11602737/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JVI.75.22.10969-10978.2001
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