Quantification of tissue-type plasminogen activator (t-PA) mRNA in human endothelial-cell cultures by hybridization with a t-PA cDNA probe.

We describe the construction of a recombinant DNA plasmid, consisting of the vector pBR322 and full-length tissue-type plasminogen-activator (t-PA) cDNA, by using polyadenylated RNA from cultured Bowes melanoma cells as substrate. A 1280-base-pair PstI restriction fragment, covering the 3' untr...

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Detalhes bibliográficos
Main Authors: van Zonneveld, A J, Chang, G T, van den Berg, J, Kooistra, T, Verheijen, J H, Pannekoek, H, Kluft, C
Formato: Artigo
Idioma:Inglês
Publicado em: 1986
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1146698/
https://ncbi.nlm.nih.gov/pubmed/3091007
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