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Chromatofocusing in the purification and separation of apo- and holo-(vitamin D-binding protein).

Chromatofocusing was used to purify the vitamin D-binding protein (DBP) from pig plasma in a procedure that consisted of an initial DEAE-cellulose chromatography followed by DEAE-Sephadex chromatography, with final purification by chromatofocusing. The protein was purified 184-fold over its concentr...

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Detalhes bibliográficos
Main Authors: Keenan, M J, Holmes, R P
Formato: Artigo
Idioma:Inglês
Publicado em: 1985
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1145109/
https://ncbi.nlm.nih.gov/pubmed/3876830
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