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Effect of Sarkosyl and heparin on single-step addition reactions catalysed by wheat-germ RNA polymerase II--poly[d(A-T)]transcription complexes.

Incubation of purified wheat-germ RNA polymerase II with poly[d(A-T)] template, Mn2+, U-A dinucleoside monophosphate primer and UTP substrate resulted in catalytic formation of the trinucleoside diphosphate U-A-U, in accordance with the results of previous studies. Both Sarkosyl and heparin inhibite...

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Bibliographic Details
Main Authors: De Mercoyrol, L, Job, C, Job, D
Format: Artigo
Language:Inglês
Published: 1989
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1138747/
https://ncbi.nlm.nih.gov/pubmed/2475103
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