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Purification and characterization of a labile rat glutathione transferase of the Mu class.

A labile GSH transferase homodimer termed 11-11 was purified from rat testis by GSH-agarose affinity chromatography followed by anion-exchange f.p.l.c. The enzyme is unstable in the absence of thiol(s) and has relatively low affinity for both 1-chloro-2,4-dinitrobenzene (Km 4.4 mM) and GSH (Km(app.)...

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Detalhes bibliográficos
Main Authors: Kispert, A, Meyer, D J, Lalor, E, Coles, B, Ketterer, B
Formato: Artigo
Idioma:Inglês
Publicado em: 1989
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1138746/
https://ncbi.nlm.nih.gov/pubmed/2764905
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