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Single amino acid substitution mutants of Klebsiella pneumoniae σ(54) defective in transcription

Transcription initiation by the σ(54) RNA polymerase requires specialised activators and their associated nucleoside triphosphate hydrolysis. To explore the roles of σ(54) in initiation we used random mutagenesis of rpoN and an in vivo activity screen to isolate functionally altered σ(54) proteins....

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Main Authors: Pitt, Melinda, Gallegos, María-Trinidad, Buck, Martin
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2000
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC113868/
https://ncbi.nlm.nih.gov/pubmed/11071928
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