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Solubilization and reconstitution of kidney 25-hydroxyvitamin D3 1 alpha- and 24-hydroxylases from vitamin D-replete pigs.

Pig kidney mitochondrial 25-hydroxyvitamin D3 1 alpha- and 24-hydroxylase activities have been solubilized with cholate/Emulgen 911 and reconstituted with NADPH, ferredoxin reductase and ferredoxin. All three of these components are required for full catalytic activity of both enzymes. Both products...

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Detalles Bibliográficos
Main Authors: Gray, R W, Ghazarian, J G
Formato: Artigo
Idioma:Inglês
Publicado: 1989
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1138545/
https://ncbi.nlm.nih.gov/pubmed/2719667
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