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Affinity of chaperonin-60 for a protein substrate and its modulation by nucleotides and chaperonin-10.

The refolding of lactate dehydrogenase fully unfolded in 4 M guanidinium chloride was initiated by dilution into assay buffer, and the emergence of active enzyme was recorded. This was performed in the presence of the following chaperonin complexes in the refolding medium: chaperonin-60 (cpn60), cpn...

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Bibliografiske detaljer
Main Authors: Staniforth, R A, Burston, S G, Atkinson, T, Clarke, A R
Format: Artigo
Sprog:Inglês
Udgivet: 1994
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1138217/
https://ncbi.nlm.nih.gov/pubmed/7912068
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