Affinity labelling of smooth-muscle myosin light-chain kinase with 5'-[p-(fluorosulphonyl)benzoyl]adenosine.

5'-(p-(Fluorosulphonyl)[14C]benzoyl)adenosine (FSBA) was synthesized and used as a probe to study the ATP-binding site of smooth-muscle myosin light-chain kinase (MLCK). FSBA modified both free MLCK and calmodulin/MLCK complex, resulting in inactivation of the kinase activity. Nearly complete p...

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Enregistré dans:
Détails bibliographiques
Auteurs principaux: Komatsu, H, Ikebe, M
Format: Artigo
Langue:Inglês
Publié: 1993
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1137654/
https://ncbi.nlm.nih.gov/pubmed/8250857
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