Expression of a biologically active plant cytochrome b5 in Escherichia coli.

Cytochrome b5 from tobacco (Nicotiana tabacum) was expressed in Escherichia coli using a T7 polymerase/promoter system as described by Studier, Rosenberg, Dunn and Dubendorff (1990) (Methods Enzymol. 185, 60-89). Transformed cells were red in colour and accumulated cytochrome b5 to a level of around...

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Detalhes bibliográficos
Main Authors: Smith, M A, Napier, J A, Stymne, S, Tatham, A S, Shewry, P R, Stobart, A K
Formato: Artigo
Idioma:Inglês
Publicado em: 1994
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1137559/
https://ncbi.nlm.nih.gov/pubmed/7945268
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