Purification of NADPH-dependent dehydroascorbate reductase from rat liver and its identification with 3 alpha-hydroxysteroid dehydrogenase.

Rat liver cytosol has been found to reduce dehydroascorbic acid (DHAA) to ascorbic acid in the presence of NADPH. The enzyme responsible for such activity has been purified by ammonium sulphate fractionation, DEAE-Sepharose, Sephadex G-100 SF and Reactive Red column chromatography, with an overall r...

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Bibliographic Details
Main Authors: Del Bello, B, Maellaro, E, Sugherini, L, Santucci, A, Comporti, M, Casini, A F
Format: Artigo
Language:Inglês
Published: 1994
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1137505/
https://ncbi.nlm.nih.gov/pubmed/7998972
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