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Identification of the vinculin-binding site in the cytoskeletal protein alpha-actinin.

Using low-speed sedimentation equilibrium we have established that vinculin binds to alpha-actinin with a Kd of 1.3 x 10(-5) M. Electron microscopy of negatively stained preparations of vinculin revealed spherical particles (diameter 11.2 nm; S.D. 1.7 nm, n = 21), whereas alpha-actinin appeared as a...

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Bibliografiske detaljer
Main Authors: McGregor, A, Blanchard, A D, Rowe, A J, Critchley, D R
Format: Artigo
Sprog:Inglês
Udgivet: 1994
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1137166/
https://ncbi.nlm.nih.gov/pubmed/8037676
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