The properties of a cloned human high-molecular-mass cytosolic phospholipase A2 investigated using a continuous fluorescence displacement assay: evidence for enzyme clustering on phospholipid vesicles.

The 85 kDa human cytosolic phospholipase A2 has been cloned and expressed in insect Sf21 cells. The pure enzyme has been investigated using a fluorescence displacement assay that provides a continuous record of phospholipid hydrolysis [Wilton (1990) Biochem. J. 266, 435-439]. The unusual kinetic pro...

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Hauptverfasser: Creaney, A, Masters, D J, Needham, M B, Gordon, R D, Mott, R, Wilton, D C
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1995
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1136599/
https://ncbi.nlm.nih.gov/pubmed/7702584
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