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A subunit interface mutant of yeast pyruvate kinase requires the allosteric activator fructose 1,6-bisphosphate for activity.

A variant form of yeast pyruvate kinase (EC 2.7.1.40) with Ser-384 mutated to proline has been engineered in order to study the allosteric properties of this enzyme. Both the mutant and wild-type enzymes were overexpressed in a strain of yeast in which the genomic copy of the pyruvate kinase gene ha...

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Bibliographic Details
Main Authors: Collins, R A, McNally, T, Fothergill-Gilmore, L A, Muirhead, H
Format: Artigo
Language:Inglês
Published: 1995
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1135862/
https://ncbi.nlm.nih.gov/pubmed/7646433
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