Over-expression and characterization of active recombinant rat liver carnitine palmitoyltransferase II using baculovirus.

The cDNA encoding rat liver carnitine palmitoyltransferase II (CPT-II) was heterologously expressed using a recombinant baculovirus/insect cell system. Unlike Escherichia coli, the baculovirus-infected insect cells expressed mostly soluble active recombinant CPT-II (rCPT-II). CPT activity from crude...

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Detalhes bibliográficos
Main Authors: Johnson, T M, Mann, W R, Dragland, C J, Anderson, R C, Nemecek, G M, Bell, P A
Formato: Artigo
Idioma:Inglês
Publicado em: 1995
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1135785/
https://ncbi.nlm.nih.gov/pubmed/7626037
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