Purification of a growth factor related to platelet-derived growth factor and a type beta transforming growth factor secreted by mouse neuroblastoma cells. A general strategy for the purification of basic polypeptide growth factors.

A general strategy was developed for the purification of basic polypeptide growth factors. This method is a combination of gel filtration, weak-cation-exchange h.p.l.c. and reverse-phase h.p.l.c., separating the proteins according to size, charge and hydrophobicity respectively. All steps are carrie...

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Bibliographic Details
Main Authors: Van den Eijnden-Van Raaij, A J, Koornneef, I, Van Oostwaard, T M, Feyen, A, Kruijer, W, De Laat, S W, Van Zoelen, E J
Format: Artigo
Language:Inglês
Published: 1989
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1135590/
https://ncbi.nlm.nih.gov/pubmed/2930456
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