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Characterization of recombinant-derived granulocyte-colony stimulating factor (G-CSF).

Human granulocyte colony-stimulating factor (G-CSF), and a mutant having a Ser for Cys substitution at residue 18 were produced in Escherichia coli strain W3110. About 60 mg of pure protein was obtained from 50 g of wet cells with a recovery of about 20%. The proteins were characterized physically a...

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Autores principales: Wingfield, P, Benedict, R, Turcatti, G, Allet, B, Mermod, J J, DeLamarter, J, Simona, M G, Rose, K
Formato: Artigo
Lenguaje:Inglês
Publicado: 1988
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1135389/
https://ncbi.nlm.nih.gov/pubmed/2464993
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