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Expression of two different forms of cDNA for thromboxane synthase in insect cells and site-directed mutagenesis of a critical cysteine residue.
cDNA coding for human placental thromboxane synthase (EC 5.3.99.5) was amplified by PCR from a human placental cDNA library and sequenced. This cDNA and a shorter cDNA isolated from a human lung cDNA library with a deletion of 163 bp near the 3' end were expressed in Spodoptera frugiperda (Sf9)...
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Main Authors: | , , , |
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格式: | Artigo |
語言: | Inglês |
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1993
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在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1134902/ https://ncbi.nlm.nih.gov/pubmed/8240243 |
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