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Calcium-induced tertiary structure modifications of endo-β-1,3-glucanase from Pyrococcus furiosus in 7.9 M guanidinium chloride

The family 16 endo-β-1,3 glucanase from the extremophilic archaeon Pyrococcus furiosus is a laminarinase, which in 7.9 M GdmCl (guanidinium chloride) maintains a significant amount of tertiary structure without any change of secondary structure. The addition of calcium to the enzyme in 7.9 M GdmCl c...

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Detalhes bibliográficos
Main Authors: Chiaraluce, Roberta, Gianese, Giulio, Angelaccio, Sebastiana, Florio, Rita, van Lieshout, Johan F. T., van der Oost, John, Consalvi, Valerio
Formato: Artigo
Idioma:Inglês
Publicado em: Portland Press Ltd. 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1134870/
https://ncbi.nlm.nih.gov/pubmed/15482259
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20041137
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