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Cloning, expression in Escherichia coli and characterization of the recombinant Neu5Acα2,6Galβ1,4GlcNAc-specific high-affinity lectin and its mutants from the mushroom Polyporus squamosus

Lectin from the mushroom Polyporus squamosus (PSL) has a unique carbohydrate-binding specificity for sialylated glycoconjugates containing Neu5Acα2,6Galβ1,4Glc/GlcNAc trisaccharide sequences of asparagine-linked glycoproteins. In the present study, we elucidate the molecular basis for its binding sp...

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Bibliografische gegevens
Hoofdauteurs: Tateno, Hiroaki, Winter, Harry C., Goldstein, Irwin J.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Portland Press Ltd. 2004
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1133824/
https://ncbi.nlm.nih.gov/pubmed/15176950
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20040391
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