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Single active-site histidine in D-xylose isomerase from Streptomyces violaceoruber. Identification by chemical derivatization and peptide mapping.

Group-specific chemical modifications of D-xylose isomerase from Streptomyces violaceruber indicated that complete loss of activity is fully correlated with the acylation of a single histidine. Active-site protection, by the ligand combination of xylitol plus Mg2+, completely blocked diethyl pyrocar...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Vangrysperre, W, Ampe, C, Kersters-Hilderson, H, Tempst, P
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1989
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1133408/
https://ncbi.nlm.nih.gov/pubmed/2604694
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