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Overexpression, purification and characterization of the Escherichia coli MelR transcription activator protein.

The gene encoding Escherichia coli MelR protein has been cloned in the expression vector pJLA502. MelR has been overexpressed, substantially purified and shown to bind to DNA fragments carrying the melAB promoter. A truncated version of the melR gene, encoding the C-terminal half of MelR, was also c...

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Detalles Bibliográficos
Main Authors: Caswell, R, Williams, J, Lyddiatt, A, Busby, S
Formato: Artigo
Idioma:Inglês
Publicado: 1992
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1133192/
https://ncbi.nlm.nih.gov/pubmed/1445207
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