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Purification and biochemical characterization of non-myristoylated recombinant pp60c-src kinase.

To obtain sufficient material for the biochemical and biophysical study of pp60c-src, we have utilized a recombinant pp60c-src baculovirus lacking the myristoylation site at codon 2. On infection of Sf9 cells, this virus produced large amounts of soluble non-myristoylated pp60c-src. The use of non-m...

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Detalhes bibliográficos
Main Authors: Lydon, N B, Gay, B, Mett, H, Murray, B, Liebetanz, J, Gutzwiller, A, Piwnica-Worms, H, Roberts, T M, McGlynn, E
Formato: Artigo
Idioma:Inglês
Publicado em: 1992
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1133104/
https://ncbi.nlm.nih.gov/pubmed/1280108
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