Dual role for protein kinase C alpha as a regulator of ion secretion in the HT29cl.19A human colonic cell line.

The involvement of protein kinase C (PKC) in the regulation of intestinal ion secretion was studied in polarized monolayers of the HT29cl.19A human colon carcinoma cell line. Carbachol, phorbol esters [PMA (phorbol 12-myristate 13-acetate) and PDB (phorbol 12,13-dibutyrate)] and 8-bromo cyclic AMP (...

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Main Authors: van den Berghe, N, Vaandrager, A B, Bot, A G, Parker, P J, de Jonge, H R
Formato: Artigo
Idioma:Inglês
Publicado em: 1992
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1132842/
https://ncbi.nlm.nih.gov/pubmed/1637359
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spelling pubmed-11328422005-09-07 Dual role for protein kinase C alpha as a regulator of ion secretion in the HT29cl.19A human colonic cell line. van den Berghe, N Vaandrager, A B Bot, A G Parker, P J de Jonge, H R Biochem J Research Article The involvement of protein kinase C (PKC) in the regulation of intestinal ion secretion was studied in polarized monolayers of the HT29cl.19A human colon carcinoma cell line. Carbachol, phorbol esters [PMA (phorbol 12-myristate 13-acetate) and PDB (phorbol 12,13-dibutyrate)] and 8-bromo cyclic AMP (8-Br-cAMP) induced Cl secretion, as measured by a rise in the short-circuit current (ISC). The electrical response to carbachol coincided with a transient translocation of PKC alpha from the soluble to the particulate fraction. The carbachol-, PDB- and 8-Br-cAMP-induced ISC responses were inhibited by pretreatment of the cells with PMA (0.5 microM) for 2 h, a time period in which PKC alpha, beta 1 and gamma levels were not changed. As shown by 86Rb+ and 125I- efflux studies, the main targets for this inhibition were basolateral K+ transporters rather than apical Cl- channels. Prolonged exposure to PMA (24 h) led to a 60% recovery of the 8-Br-cAMP response, but not of the carbachol- or PDB-provoked secretion. As shown by immunoblotting with PKC-isoenzyme-specific antisera, the recovery of the 8-Br-cAMP response coincided with the down-regulation of PKC alpha, whereas the levels of PKC beta 1 and gamma were unmodified. These results suggest that PKC alpha, but not PKC beta 1 or gamma, is involved in both acute stimulation and chronic inhibition of ion secretion in the HT29cl.19A colonic cell line. 1992-07-15 /pmc/articles/PMC1132842/ /pubmed/1637359 Text en
institution US NLM
collection PubMed Central
language Inglês
format Artigo
topic Research Article
spellingShingle Research Article
van den Berghe, N
Vaandrager, A B
Bot, A G
Parker, P J
de Jonge, H R
Dual role for protein kinase C alpha as a regulator of ion secretion in the HT29cl.19A human colonic cell line.
description The involvement of protein kinase C (PKC) in the regulation of intestinal ion secretion was studied in polarized monolayers of the HT29cl.19A human colon carcinoma cell line. Carbachol, phorbol esters [PMA (phorbol 12-myristate 13-acetate) and PDB (phorbol 12,13-dibutyrate)] and 8-bromo cyclic AMP (8-Br-cAMP) induced Cl secretion, as measured by a rise in the short-circuit current (ISC). The electrical response to carbachol coincided with a transient translocation of PKC alpha from the soluble to the particulate fraction. The carbachol-, PDB- and 8-Br-cAMP-induced ISC responses were inhibited by pretreatment of the cells with PMA (0.5 microM) for 2 h, a time period in which PKC alpha, beta 1 and gamma levels were not changed. As shown by 86Rb+ and 125I- efflux studies, the main targets for this inhibition were basolateral K+ transporters rather than apical Cl- channels. Prolonged exposure to PMA (24 h) led to a 60% recovery of the 8-Br-cAMP response, but not of the carbachol- or PDB-provoked secretion. As shown by immunoblotting with PKC-isoenzyme-specific antisera, the recovery of the 8-Br-cAMP response coincided with the down-regulation of PKC alpha, whereas the levels of PKC beta 1 and gamma were unmodified. These results suggest that PKC alpha, but not PKC beta 1 or gamma, is involved in both acute stimulation and chronic inhibition of ion secretion in the HT29cl.19A colonic cell line.
author van den Berghe, N
Vaandrager, A B
Bot, A G
Parker, P J
de Jonge, H R
author_facet van den Berghe, N
Vaandrager, A B
Bot, A G
Parker, P J
de Jonge, H R
author_sort van den Berghe, N
title Dual role for protein kinase C alpha as a regulator of ion secretion in the HT29cl.19A human colonic cell line.
title_short Dual role for protein kinase C alpha as a regulator of ion secretion in the HT29cl.19A human colonic cell line.
title_full Dual role for protein kinase C alpha as a regulator of ion secretion in the HT29cl.19A human colonic cell line.
title_fullStr Dual role for protein kinase C alpha as a regulator of ion secretion in the HT29cl.19A human colonic cell line.
title_full_unstemmed Dual role for protein kinase C alpha as a regulator of ion secretion in the HT29cl.19A human colonic cell line.
title_sort dual role for protein kinase c alpha as a regulator of ion secretion in the ht29cl.19a human colonic cell line.
publishDate 1992
url https://ncbi.nlm.nih.gov/pmc/articles/PMC1132842/
https://ncbi.nlm.nih.gov/pubmed/1637359
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