Purification and characterization of the blue-green rat phaeochromocytoma (PC12) tyrosine hydroxylase with a dopamine-Fe(III) complex. Reversal of the endogenous feedback inhibition by phosphorylation of serine-40.

Tyrosine hydroxylase (TH) was purified from tumours of rat phaeochromocytoma (PC12) cells by a three-step purification procedure giving 30 mg of pure enzyme in 3 days. The enzyme sedimented with an S(eo),w value of 9.2 S and revealed an apparent subunit molecular mass of 62 kDa with a minor 60 kDa c...

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Detalhes bibliográficos
Main Authors: Andersson, K K, Vassort, C, Brennan, B A, Que, L, Haavik, J, Flatmark, T, Gros, F, Thibault, J
Formato: Artigo
Idioma:Inglês
Publicado em: 1992
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Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1132593/
https://ncbi.nlm.nih.gov/pubmed/1352446
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