Secretion by overexpression and purification of the water-soluble Streptomyces K15 DD-transpeptidase/penicillin-binding protein.

Though synthesized with a cleavable signal peptide and devoid of membrane anchors, the 262-amino-acid-residue Streptomyces K15 DD-transpeptidase/penicillin-binding protein is membrane-bound. Overexpression in Streptomyces lividans resulted in the export of an appreciable amount of the synthesized pr...

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Detalhes bibliográficos
Main Authors: Palomeque-Messia, P, Quittre, V, Leyh-Bouille, M, Nguyen-Distèche, M, Gershater, C J, Dacey, I K, Dusart, J, Van Beeumen, J, Ghuysen, J M
Formato: Artigo
Idioma:Inglês
Publicado em: 1992
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1132083/
https://ncbi.nlm.nih.gov/pubmed/1445284
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