Expression of active octameric chicken cardiac mitochondrial creatine kinase in Escherichia coli.

Sarcomeric mitochondrial creatine kinase (Mib-CK) of chicken was expressed in Escherichia coli as a soluble enzyme by using an inducible phage-T7 promoter. Up to one third of the protein in E. coli extracts consisted of soluble recombinant Mib-CK in an enzymically active form. Approx. 20 mg of nearl...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Furter, R, Kaldis, P, Furter-Graves, E M, Schnyder, T, Eppenberger, H M, Wallimann, T
Formato: Artigo
Lenguaje:Inglês
Publicado: 1992
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1131953/
https://ncbi.nlm.nih.gov/pubmed/1471992
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!

Ejemplares similares