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Efficient gene targeted random mutagenesis in genetically stable Escherichia coli strains

We describe a method to generate in vivo collections of mutants orders of magnitude larger than previously possible. The method favors accumulation of mutations in the target gene, rather than in the host chromosome. This is achieved by propagating the target gene on a plasmid, in Escherichia coli c...

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Autors principals: Fabret, Céline, Poncet, Sandrine, Danielsen, Steffen, Borchert, Torben V., Ehrlich, S. Dusko, Jannière, Laurent
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2000
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC113164/
https://ncbi.nlm.nih.gov/pubmed/11058145
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