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Overexpression of restructured pyruvate dehydrogenase complexes and site-directed mutagenesis of a potential active-site histidine residue.

The aceEF-lpd operon of Escherichia coli encodes the pyruvate dehydrogenase (E1p), dihydrolipoamide acetyltransferase (E2p) and dihydrolipoamide dehydrogenase (E3) components of the pyruvate dehydrogenase multienzyme complex (PDH complex). A thermoinducible expression system was developed to amplify...

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Main Authors: Russell, G C, Guest, J R
格式: Artigo
語言:Inglês
出版: 1990
主題:
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC1131597/
https://ncbi.nlm.nih.gov/pubmed/2201286
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