Purification and properties of acetyl-CoA synthetase from Bradyrhizobium japonicum bacteroids.

Acetyl-CoA synthetase was purified 800-fold from Bradyrhizobium japonicum bacteroids. A specific activity of 16 mumol/min per mg of protein was achieved, with a 30-40% yield. The purification scheme consisted of only three consecutive chromatography steps. The enzyme has a native Mr of 150,000, esti...

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Bibliografske podrobnosti
Main Authors: Preston, G G, Wall, J D, Emerich, D W
Format: Artigo
Jezik:Inglês
Izdano: 1990
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1131261/
https://ncbi.nlm.nih.gov/pubmed/1970239
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