Dependence of the P2-S2 stereochemical selectivity of papain on the nature of the catalytic-site chemistry. Quantification of selectivity in the catalysed hydrolysis of the enantiomeric N-acetylphenylalanylglycine 4-nitroanilides.

1. N-Acetyl-L-phenylalanylglycine 4-nitroanilide and its D-enantiomer were synthesized and characterized and used as substrates with which to evaluate stereochemical selectivity in papain (EC 3.4.22.2)-catalysed hydrolysis. 2. Kinetic analysis at pH 6.0, I 0.1, 8.3% (v/v) NN-dimethylformamide and 25...

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Detalles Bibliográficos
Main Authors: Kowlessur, D, Thomas, E W, Topham, C M, Templeton, W, Brocklehurst, K
Formato: Artigo
Idioma:Inglês
Publicado: 1990
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1131189/
https://ncbi.nlm.nih.gov/pubmed/2327954
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