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The synthesis, kinetic characterization and application of biotinylated aminoacylchloromethanes for the detection of chymotrypsin and trypsin-like serine proteinases.

The synthesis of two biotinylated affinity labels for chymotrypsin and trypsin-like serine proteinases is described, along with their kinetic characterization and application to the detection of these proteinases after PAGE and Western blotting. Thus the chloromethane analogues biotinylphenylalanylc...

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Detalhes bibliográficos
Main Authors: Kay, G, Bailie, J R, Halliday, I M, Nelson, J, Walker, B
Formato: Artigo
Idioma:Inglês
Publicado em: 1992
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1131056/
https://ncbi.nlm.nih.gov/pubmed/1575691
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