Structure-function analysis of human transforming growth factor-alpha by site-directed mutagenesis.

Site-directed mutants of transforming growth factor-alpha (TGF-alpha) were expressed in an Escherichia coli outer membrane protein A (ompA) expression/secretion vector under the transcriptional control of the lambda PL promoter. TGF-alpha mutant proteins were isolated from cell pellets using alkalin...

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Bibliographic Details
Main Authors: Feild, J A, Reid, R H, Rieman, D J, Kline, T P, Sathe, G, Greig, R G, Anzano, M A
Format: Artigo
Language:Inglês
Published: 1992
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1130998/
https://ncbi.nlm.nih.gov/pubmed/1567383
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