Glycosyl-phosphatidylinositol-anchored membrane proteins can be distinguished from transmembrane polypeptide-anchored proteins by differential solubilization and temperature-induced phase separation in Triton X-114.

Treatment of kidney microvillar membranes with the non-ionic detergent Triton X-114 at 0 degrees C, followed by low-speed centrifugation, generated a detergent-insoluble pellet and a detergent-soluble supernatant. The supernatant was further fractionated by phase separation at 30 degrees C into a de...

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Bibliografische gegevens
Hoofdauteurs: Hooper, N M, Bashir, A
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1991
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Research Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1130517/
https://ncbi.nlm.nih.gov/pubmed/1837216
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