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Triplet nucleotide removal at random positions in a target gene: the tolerance of TEM-1 β-lactamase to an amino acid deletion
The deletion of amino acids is one of the evolutionary mechanisms by which nature adapts the function of proteins. A simple method has been developed that mimics this event in vitro by introducing a deletion of exactly three nucleotides at random positions in a target gene. The method involved the e...
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| Formato: | Artigo |
| Idioma: | Inglês |
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Oxford University Press
2005
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1129029/ https://ncbi.nlm.nih.gov/pubmed/15897323 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni077 |
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