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Functional Analysis of the Interaction between VPg-Proteinase (NIa) and RNA Polymerase (NIb) of Tobacco Etch Potyvirus, Using Conditional and Suppressor Mutants

The tobacco etch potyvirus (TEV) RNA-dependent RNA polymerase (NIb) has been shown to interact with the proteinase domain of the VPg-proteinase (NIa). To investigate the significance of this interaction, a Saccharomyces cerevisiae two-hybrid assay was used to isolate conditional NIa mutant proteins...

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Detalles Bibliográficos
Main Authors: Daròs, José-Antonio, Schaad, Mary C., Carrington, James C.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 1999
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC112894/
https://ncbi.nlm.nih.gov/pubmed/10482627
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