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Lentivirus Gene Transfer in Murine Hematopoietic Progenitor Cells Is Compromised by a Delay in Proviral Integration and Results in Transduction Mosaicism and Heterogeneous Gene Expression in Progeny Cells

Human immunodeficiency virus type 1-based lentivirus vectors containing the green fluorescent protein (GFP) gene were used to transduce murine Lin(−) c-kit(+) Sca1(+) primitive hematopoietic progenitor cells. Following transduction, the cells were plated into hematopoietic progenitor cell assays in...

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Autors principals: Mikkola, Hanna, Woods, Niels-Bjarne, Sjögren, Marketa, Helgadottir, Hildur, Hamaguchi, Isao, Jacobsen, Sten-Eirik, Trono, Didier, Karlsson, Stefan
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2000
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC112474/
https://ncbi.nlm.nih.gov/pubmed/11090191
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