Generation of Dominant Selectable Markers for Resistance to Pseudomonic Acid by Cloning and Mutagenesis of the ileS Gene from the Archaeon Methanosarcina barkeri Fusaro

Currently, only one selectable marker is available for genetic studies in the archaeal genus Methanosarcina. Here we report the generation of selectable markers that encode resistance to pseudomonic acid (PA(r)) in Methanosarcina species by mutagenesis of the isoleucyl-tRNA synthetase gene (ileS) fr...

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Pubblicato in:	J Bacteriol
Autori principali:	Boccazzi, Paolo, Zhang, Jun Kai, Metcalf, William W.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2000
Soggetti:	Genetics and Molecular Biology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC111328/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10762266/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.182.9.2611-2618.2000
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