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Efficient Targeted Mutagenesis in Borrelia burgdorferi

Genetic studies in Borrelia burgdorferi have been hindered by the lack of a nonborrelial selectable marker. Currently, the only selectable marker is gyrB(r), a mutated form of the chromosomal gyrB gene that encodes the B subunit of DNA gyrase and confers resistance to the antibiotic coumermycin A(1)...

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Autori principali: Bono, James L., Elias, Abdallah F., Kupko, John J., Stevenson, Brian, Tilly, Kit, Rosa, Patricia
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2000
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC111306/
https://ncbi.nlm.nih.gov/pubmed/10762244
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