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Construction and Characterization of a recA Mutant of Thiobacillus ferrooxidans by Marker Exchange Mutagenesis

To construct Thiobacillus ferrooxidans mutants by marker exchange mutagenesis, a genetic transfer system is required. The transfer of broad-host-range plasmids belonging to the incompatibility groups IncQ (pKT240 and pJRD215), IncP (pJB3Km1), and IncW (pUFR034) from Escherichia coli to two private T...

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Bibliografiske detaljer
Main Authors: Liu, Zhenying, Guiliani, Nicolas, Appia-Ayme, Corinne, Borne, Françoise, Ratouchniak, Jeanine, Bonnefoy, Violaine
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2000
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC111277/
https://ncbi.nlm.nih.gov/pubmed/10735871
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