Protein Engineering of the Archetypal Nitroarene Dioxygenase of Ralstonia sp. Strain U2 for Activity on Aminonitrotoluenes and Dinitrotoluenes through Alpha-Subunit Residues Leucine 225, Phenylalanine 350, and Glycine 407

Naphthalene dioxygenase (NDO) from Ralstonia sp. strain U2 has not been reported to oxidize nitroaromatic compounds. Here, saturation mutagenesis of NDO at position F350 of the α-subunit (NagAc) created variant F350T that produced 3-methyl-4-nitrocatechol from 2,6-dinitrotoluene (26DNT), that releas...

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Dades bibliogràfiques
Autors principals: Keenan, Brendan G., Leungsakul, Thammajun, Smets, Barth F., Mori, Masa-aki, Henderson, David E., Wood, Thomas K.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2005
Matèries:
Enzymes and Proteins
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1112016/
https://ncbi.nlm.nih.gov/pubmed/15866914
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.187.10.3302-3310.2005
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