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Role of the RNA Polymerase α Subunits in MetR-Dependent Activation of metE and metH: Important Residues in the C-Terminal Domain and Orientation Requirements within RNA Polymerase

Many transcription factors activate by directly interacting with RNA polymerase (RNAP). The C terminus of the RNAP α subunit (αCTD) is a common target of activators. We used both random mutagenesis and alanine scanning to identify αCTD residues that are crucial for MetR-dependent activation of metE...

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Bibliographische Detailangaben
Hauptverfasser: Fritsch, Paula S., Urbanowski, Mark L., Stauffer, George V.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2000
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC110999/
https://ncbi.nlm.nih.gov/pubmed/10986259
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