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Binding of Ferric Enterobactin by the Escherichia coli Periplasmic Protein FepB

The periplasmic protein FepB of Escherichia coli is a component of the ferric enterobactin transport system. We overexpressed and purified the binding protein 23-fold from periplasmic extracts by ammonium sulfate precipitation and chromatographic methods, with a yield of 20%, to a final specific act...

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Bibliografiske detaljer
Udgivet i:J Bacteriol
Principais autores: Sprencel, Cathy, Cao, Zhenghua, Qi, Zengbiao, Scott, Daniel C., Montague, Marjorie A., Ivanoff, Nora, Xu, Jide, Raymond, Kenneth M., Newton, Salete M. C., Klebba, Phillip E.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2000
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC110977/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10986237/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.182.19.5359-5364.2000
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